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Clinical Trial Details
Impact of aging on gene expression and RNA splice variants in peripheral blood cells
Clinical Trial ( IRB ) #: 14-194J-3
Title: Impact of aging on gene expression and RNA splice variants in peripheral blood cells
Principal Investigator: Dr. George Kuchel
Description: The primary objective of this IRB proposal is to obtain preliminary feasibility and pilot data for the submission of a competitive renewal this fall for Dr Palucka's HIPC U19 grant currently funded by NIH. PRIMARY HYPOTHESIS We will be able to gain a comprehensive view of the genomic alterations associated with aging and frailty using immunological and system biology approaches. Furthermore, we theorize that these changes will be associated with specific microbiome composition. SPECIFIC AIMS This is a single-center exploratory study to carry out transcriptional and microbiome profiling analysis to gain a comprehensive view of genomic alterations associated with aging and frailty. Aim 1: To collect the samples from well-characterized volunteer cohorts. Aim 2: To establish whole blood isoform profiles. Aim 3: To establish epigenetic profiles of PBMCs and sorted blood cells Aim 4: To characterize microbiome in saliva and stool samples. SIGNIFICANCE It becomes critically important to apply system biology approaches and in-depth genomics to understand aging and related alterations. Identifying the immunologic parameters that correlate with or predict immune alterations will have a significant impact on the increased understanding of disease physiology in older age and eventually novel biomarkers and targets for therapy. RELEVANCE Recent technological breakthroughs have made the study of biological systems on a large scale a reality, thus offering unprecedented opportunities to comprehensively profile genome and immune responses in human subjects. A major challenge when engaging in such studies is to establish baseline values in subjects over time under "healthy" conditions. This data will be used as a basis for design of futures studies identifying changes occurring in response to challenge as for example acute infection or vaccination.
Classification:
  Aging
  Healthy Volunteer
Eligibility Criteria: Check with study contact
How to Contact: Nancy Dean. Telephone: (860) 679-8865. Email: Ndean@nso1.uchc.edu or Lisa Kenyon-Pesce. Telephone: (860) 679-2305. Email: Kenyon-Pesce@uchc.edu
Enrollment Status/Comments: The study is finished and is no longer enrolling/recruiting.